Expression changes of NaV channel subunits correlate with developmental maturation of electrophysiological characteristics of rat cerebellar Purkinje neurons / 南方医科大学学报

OBJECTIVE@#To investigate the variations in the expression of voltage-gated sodium (Nav) channel subunits during development of rat cerebellar Purkinje neurons and their correlation with maturation of electrophysiological characteristics of the neurons.@*METHODS@#We observed the changes in the expression levels of NaV1.1, 1.2, 1.3 and 1.6 during the development of Purkinje neurons using immunohistochemistry in neonatal (5-7 days after birth), juvenile (12-14 days), adolescent (21-24 days), and adult (42-60 days) SD rats. Using whole-cell patch-clamp technique, we recorded the spontaneous electrical activity of the neurons in ex vivo brain slices of rats of different ages to analyze the changes of electrophysiological characteristics of these neurons during development.@*RESULTS@#The expression of NaV subunits in rat cerebellar Purkinje neurons showed significant variations during development. NaV1.1 subunit was highly expressed throughout the developmental stages and increased progressively with age (P < 0.05). NaV1.2 expression was not detected in the neurons in any of the developmental stages (P > 0.05). The expression level of NaV1.3 decreased with development and became undetectable after adolescence (P < 0.05). NaV1.6 expression was not detected during infancy, but increased with further development (P < 0.05). NaV1.1 and NaV1.3 were mainly expressed in the early stages of development. With the maturation of the rats, NaV1.3 expression disappeared and NaV1.6 expression increased in the neurons. NaV1.1 and NaV1.6 were mainly expressed after adolescence. The total NaV protein level increased gradually with development (P < 0.05) and tended to stabilize after adolescence. The spontaneous frequency and excitability of the Purkinje neurons increased gradually with development and reached the mature levels in adolescence. The developmental expression of NaV subunits was positively correlated with discharge frequency (r=0.9942, P < 0.05) and negatively correlated with the excitatory threshold of the neurons (r=0.9891, P < 0.05).@*CONCLUSION@#The changes in the expression levels of NaV subunits are correlated with the maturation of high frequency electrophysiological properties of the neurons, suggesting thatmature NaV subunit expressions is the basis of maturation of electrophysiological characteristics of the neurons.

Persistencia de epónimos en terminologia histologica / Eponyms persistence in terminologia histologica

El uso de epónimos aún es una práctica frecuentemente utilizada entre médicos clínicos y académicos para referirse a las distintas estructuras en histología. A pesar de los esfuerzos por parte de la comunidad morfológica por desarraigarlos del lenguaje médico, hoy en día se encuentran, inclusive presentes en Terminologia Histologica, tal como en los casos de Schannocytus (H2.00.06.2.02003) referente a la Célula de Schwann; Complexus golgiensis (H1.00.01.3.0146) referente al Aparato de Golgi, Cellula panethensis (H3.04.03.0.00017) referente a la Célula de Paneth, y Neuron purkinjense (H3.11.03.4.01015) referente a la Neurona de Purkinje, que aluden a los investigadores Theodor Schwann, Camillo Golgi, Joseph Paneth y Jan Evangelista Purkinje, respectivamente. El objetivo del presente estudio fue realizar un análisis de los términos antes nombrados desde un punto de vista lingüístico y proponer nuevas denominaciones, siguiendo los parámetros establecidos en la Terminología, en la cual los nombres de las estructuras deben tener un valor informativo, estar escritos en latín como lengua base y eliminar el uso de los epónimos. Los términos analizados, se refieren a nombres de células u organelos frecuentemente utilizados en textos educativos, sin embargo, son poco descriptivos, muchos de ellos con raíces netamente griegas y otros neologismos, cuyas denominaciones, por consenso y en honor a investigadores connotados han perdurado en el tiempo. Proponemos modificaciones con respecto a su denominación, así como a sus derivados, utilizando términos procedentes del latín. En resumen, pretendemos que con estos antecedentes iniciales puedan entregarse argumentos que permitan seguir unificando criterios y que ellos puedan ser considerados por los expertos que conforman el Programa Federativo Internacional de Terminología Anatómica y, como bien se señala, permitir el establecimiento de diálogo con los miembros de la Federación Internacional de Asociaciones de Anatomistas e ir mejorando la comunicación científica entre los diferentes actores de las ciencias morfológicas.

Eponyms are still frequently used among clinicians and scholars to refer to the various structures in histology. Despite efforts by the morphological community to eradicate eponyms from medical language, nowadays they are practical, and even present in Terminologia Histologica (TH), such as in the case of Schannocytus (H2.00.06.2.02003) concerning the term Schwann cell; Complexus golgiensis (H1.00.01.3.0146) relating to the Golgi apparatus, Cellula panethensis (H3.04.03.0.00017) concerning the Paneth cell and Neuron purkinjense (H3.11.03.4.01015), the term Purkinje neuron which refers to researchers Theodor Schwann, Camillo Golgi, Joseph Paneth and Jan Evangelist Purkinje, respectively. The aim of this study was to conduct an analysis of these terms from a linguistic point of view and propose new Latin names, following guidelines established in the terminology wherein the names of structures must, have an informative value, be written in Latin as a base language, and eliminate the use of eponyms. The terms analyzed, refer to cells or organelles names frequently used, they have limited descriptive value, many with purely Greek roots and other neologisms, which names have endured over time in honor of renowned researchers. Using terms from Larin, we propose modifications with respect to classification and derivatives. In conclusion, we hope that with this introduction, the information to consolidate standards will be considered by the experts of the Federal International Committee on Anatomical Terminology and further, initiate a dialogue with International Federation of Associations of Anatomists members, while encouraging ongoing communication between the various players of morphological sciences.